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Fire needling acupuncture inhibits <t>TLR4/MyD88/NF-κB/LCN2</t> pathway. ( A ) Representative Western blots showing TLR4, MyD88, NF-κB, p-NF-κB, and LCN2 in the epidermis of psoriasis-like skin lesions. ( B – E ) Comparison of TLR4, MyD88, p-NF-κB, and LCN2 protein levels between different groups (n = 3 for each group). ### P < 0.001 vs Control; * P < 0.05, ** P < 0.01 vs IMQ.
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Fire needling acupuncture inhibits <t>TLR4/MyD88/NF-κB/LCN2</t> pathway. ( A ) Representative Western blots showing TLR4, MyD88, NF-κB, p-NF-κB, and LCN2 in the epidermis of psoriasis-like skin lesions. ( B – E ) Comparison of TLR4, MyD88, p-NF-κB, and LCN2 protein levels between different groups (n = 3 for each group). ### P < 0.001 vs Control; * P < 0.05, ** P < 0.01 vs IMQ.
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primary antibodies against toll  (Proteintech)
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Fire needling acupuncture inhibits <t>TLR4/MyD88/NF-κB/LCN2</t> pathway. ( A ) Representative Western blots showing TLR4, MyD88, NF-κB, p-NF-κB, and LCN2 in the epidermis of psoriasis-like skin lesions. ( B – E ) Comparison of TLR4, MyD88, p-NF-κB, and LCN2 protein levels between different groups (n = 3 for each group). ### P < 0.001 vs Control; * P < 0.05, ** P < 0.01 vs IMQ.
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Fire needling acupuncture inhibits TLR4/MyD88/NF-κB/LCN2 pathway. ( A ) Representative Western blots showing TLR4, MyD88, NF-κB, p-NF-κB, and LCN2 in the epidermis of psoriasis-like skin lesions. ( B – E ) Comparison of TLR4, MyD88, p-NF-κB, and LCN2 protein levels between different groups (n = 3 for each group). ### P < 0.001 vs Control; * P < 0.05, ** P < 0.01 vs IMQ.

Journal: Journal of Inflammation Research

Article Title: Fire Needling Acupuncture Reduces Neutrophil Extracellular Traps (NETs) Formation and Inhibits TLR4/NF-κB/LCN2 Pathway in Psoriasis-Like Skin Lesions

doi: 10.2147/JIR.S569462

Figure Lengend Snippet: Fire needling acupuncture inhibits TLR4/MyD88/NF-κB/LCN2 pathway. ( A ) Representative Western blots showing TLR4, MyD88, NF-κB, p-NF-κB, and LCN2 in the epidermis of psoriasis-like skin lesions. ( B – E ) Comparison of TLR4, MyD88, p-NF-κB, and LCN2 protein levels between different groups (n = 3 for each group). ### P < 0.001 vs Control; * P < 0.05, ** P < 0.01 vs IMQ.

Article Snippet: After blocking nonspecific binding sites, primary antibodies against TLR4 (1:4000; Proteintech, USA), MyD88 (1:10000; Proteintech, USA), NF-κB (1:1000; Proteintech, USA), p-NF-κB (1:1000; CST, USA), LCN2 (1:1000; Proteintech, USA), and β-actin (1:1000; Proteintech, USA) were added, and PVDF membranes were incubated overnight at 4°C.

Techniques: Western Blot, Comparison, Control

Fire needling acupuncture reduces NETs formation and inhibits TLR4/MyD88/NF-κB/LCN2 pathway in psoriasis (the red arrows indicate inhibition).

Journal: Journal of Inflammation Research

Article Title: Fire Needling Acupuncture Reduces Neutrophil Extracellular Traps (NETs) Formation and Inhibits TLR4/NF-κB/LCN2 Pathway in Psoriasis-Like Skin Lesions

doi: 10.2147/JIR.S569462

Figure Lengend Snippet: Fire needling acupuncture reduces NETs formation and inhibits TLR4/MyD88/NF-κB/LCN2 pathway in psoriasis (the red arrows indicate inhibition).

Article Snippet: After blocking nonspecific binding sites, primary antibodies against TLR4 (1:4000; Proteintech, USA), MyD88 (1:10000; Proteintech, USA), NF-κB (1:1000; Proteintech, USA), p-NF-κB (1:1000; CST, USA), LCN2 (1:1000; Proteintech, USA), and β-actin (1:1000; Proteintech, USA) were added, and PVDF membranes were incubated overnight at 4°C.

Techniques: Inhibition